Products / pH Indicators


C.A.S. No. 76-60-8
M.F. C21H14Br4O5S
M.W. 698.04gm/mole


Dye Content (by Spectrophotometry) >95%
Transition Range pH 3.8-5.4
Greenish Yellow-Blue
Solubility 0.1% (95% EtOH) Clear solution
Absorption maximum (pH 3.8) ?1max 438-443nm
Absorption maximum (pH 5.4) ?2max 615-618nm
Absorptivity (A1%/1cm at pH 3.8 at ?1max) 240-270
Absorptivity (A1%/1cm at pH 5.4 at ?2max) 530-570
Loss On Drying (110°C) <3%

Chemical Name

3,3-Bis(3,5- dibromo-4- hydroxy-2- methylphenyl)-
3H-benzo[c][1,2]oxathiole 1,1-dioxide

InChI Key


Product Description

Bromocresol Green is a dye of the triphenylmethane family (triarylmethane dyes). It binds quantitatively with human albumin forming an intense blue-green complex with an absorbance maximum at 628 nm.
Format Powder
pH 3.8 - 5.4, yellow to blue(Lit.)


Soluble in water (6 mg/mL), ethanol (40 mg/mL) or methyl cellosolve (200 mg/mL).
Storage and Handling: Store at Room Temperature(15- 30 °C).
Density : ~2.1 g/cm 3 (Predicted)
pK a : 4.24 (Predicted)


Bromocresol Green is useful in a variety of colorimetric detection technologies. It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge transfer complexation processes. In TLC, it is used for visualisation of the compounds with functional groups whose pKa is below 5.0. Bromocresol green is a pH indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.It is a pH sensitive triphenylmethane dye useful in various colorimetric detection studies and as an activity tracking dye for DNA agarose gel electrophoresis. It is also used in TLC (thin-layer chromatography) for visualizing compounds containing functional groups having pKa less than 5.

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