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AZURE B (AZURE I) Print Download

C.A.S. No. 531-55-5
CI NO. 52010
M.F. C15H16CIN3S
M.W. 305.83gm/mole


Dye Content (by Spectrophotometry) 80%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist. Water) ?max 645-650nm
Absorptivity (A1%/1cm,in water at ?max on dried substance) >1600
Loss On Drying (110°C) <5%
Suitability for microscopy Passes test

Chemical Name

3-(Dimethylamino)-7-(methylamino)phenothiazin-5-ium chloride

InChI Key



Soluble in water; slightly soluble in ethanol, methanol.

Product Description:

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.Azure B has been used for the staining of chromosomes. Azure B has been used in M'Fadyean stain to visualize Bacillus anthracis capsule.


Azure B is used for differentiating cellular RNA (purplish) and DNA (blue-green) in plant tissues. Useful as a stain for Negri Bodies. Can be used in the Romanowsky-type stains for blood and blood protozoa. Purified azure B, on the other hand, gave reproducibly stained, deposit-free preparations. Reticulocyte counts obtained from azure B preparations correlated almost exactly with those determined using new methylene blue. Purified azure B is therefore recommended as a convenient reticulocyte stain for routine use.

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