Products / pH Indicators

NILE BLUE CHLORIDE Print Download

C.A.S. No. 2381-85-3
CI NO. 51180
M.F. C20H20ClN3O
M.W. 353.85gm/mole

Specifications:

Dye Content (by Spectrophotometry) >90%
Solubility 0.1% (50% EtOH) Clear solution
Absorption maximum (50% EtOH) ?max 635-639nm
Absorptivity (A1%/1cm in 50% EtOH, at ?max) >1800
Loss On Drying (110°C) <8%

Chemical name

Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) chloride

InChI Key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

Solubility

Soluble in ethyl alcohol

Product Description:

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies. Biochem/physiol Actions. Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0).

Applications:

Nile Blue chloride, or Nile Blue A, is a fluorescence reference standard used for various histologic staining. It is commonly utilized in studies involved with lipid and nuclei staining. Nile Blue chloride imparts a pink color to unsaturated glycerides and neutral lipids and stains nuclei and acids (such as fatty acids, chormolipids and phospholipids) a purple-blue color.

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