Products / pH Indicators

BROMOPHENOL BLUE (ACS GRADE) Print Download

C.A.S. No. 115-39-9
M.F. C19H10Br4O5S
M.W. 669.99gm/mole

Specifications:

Dye Content (by Spectrophotometry) >95%
Transition Range pH 3.0-4.6
Greenish Yellow-Blue
Solubility 0.1% (95% EtOH) Clear solution
Absorption maximum (pH 3.0) ?1max 434-439nm
Absorption maximum (pH 4.6) ?2max 590-593nm
Absorptivity (A1%/1cm at pH 3.0 at ?1max) 350-385
Absorptivity (A1%/1cm at pH 4.6 at ?2max) 940-1000
Loss On Drying (110°C) <1%

Chemical Name

Phenol, 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis[2,6-dibromo-, S,S-dioxide

InChI Key

UDSAIICHUKSCKT-UHFFFAOYSA-N

Solubility

Soluble in acetic acid.Soluble in water (about 0.4 g/100 mL); more soluble in methyl and ethyl alcohol, and in benzene. Freely soluble in NaOH solutions with the formation of a water-soluble sodium salt.In water, 3 mg/mL

Product Description:

Bromophenol blue is an acid-base indicator since its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein (a popular indicator). Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis

Applications:

Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Concentrations of bromophenol blue (I) in plasma, urine, and bile were determined spectrophotometrically after intravenous bolus injections and infusions in rats. The plasma concentrations were found to decrease mono exponentially after all doses except the highest, where the decrease was biexponential. Bromophenol blue (BPB) is the most widely used stain in gel electrophoresis for tracing the migration of samples on electrophoretic gels. If the gel is staind with 1 - 5 mg bromophenol blue, this may be detected much easier and earlier. In case that the stain will interfere with other applications (e. g. silver staining), a pre-run of the gel will remove bromophenol blue. It does not influence the migration of the samples. Add the solid stain before adding the catalyst.

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